INTRACELLULAR DELIVERY OF PROTEIN-KINASE C-ALPHA OR C-EPSILON ISOFORM-SPECIFIC ANTIBODIES PROMOTES ACQUISITION OF A MORPHOLOGICALLY DIFFERENTIATED PHENOTYPE IN NEUROBLASTOMA-CELLS

被引：52

作者：

LELI, U

PARKER, PJ

SHEA, TB

机构：

[1] HARVARD UNIV, MCLEAN HOSP, SCH MED, MOLEC NEUROSCI LAB, BELMONT, MA 02178 USA

[2] HARVARD UNIV, CELLULAR & DEV NEUROBIOL LAB, BELMONT, MA 02178 USA

[3] HARVARD UNIV, DEPT PSYCHIAT, BELMONT, MA 02178 USA

[4] IMPERIAL CANC RES FUND, LONDON WC2A 3PX, ENGLAND

来源：

FEBS LETTERS | 1992年 / 297卷 / 1-2期

关键词：

DIFFERENTIATION; ISOFORM; NEUROBLASTOMA; NEURITOGENESIS; PROTEIN KINASE;

D O I：

10.1016/0014-5793(92)80334-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The protein kinase C (PKC) family participates in a ubiquitous cell signalling system utilizing increased turnover of phosphoinositides. Because down-regulation of total PKC activity has been implicated in the acquisition of a morphologically differentiated phenotype in SH-SY5Y neuroblastoma cells, we aimed to identify the specific PKC isoforms in this process. Here we report that intracellular delivery of PKC-alpha and -epsilon, but not -beta, -gamma or -delta isoform-specific antibodies is sufficient to induce acquisition of a morphologically differentiated phenotype in SH-SY5Y neuroblastoma cells.

引用

页码：91 / 94

页数：4

共 35 条

[1] FUNCTIONAL-DIFFERENTIATION OF A HUMAN GANGLION-CELL DERIVED NEURO-BLASTOMA CELL-LINE SH-SY5Y INDUCED BY A PHORBOL ESTER (TPA) [J].