INDUCTION OF HEME OXYGENASE BY DELTA-12-PROSTAGLANDIN-J2 IN PORCINE AORTIC ENDOTHELIAL-CELLS

被引：33

作者：

KOIZUMI, T

NEGISHI, M

ICHIKAWA, A

机构：

[1] Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Kyoto

来源：

PROSTAGLANDINS | 1992年 / 43卷 / 02期

关键词：

D O I：

10.1016/0090-6980(92)90081-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DELTA(12)-Prostaglandin (PG)J2 stimulated the synthesis of a 31,000-dalton protein (termed p31) and the induction of cellular heme oxygenase activity in porcine aortic endothelial cells. A good correlation was observed between the time courses and dose dependencies of the induction of p31 synthesis and that of heme oxygenase activity by DELTA(12)-PGJ2. Hemin, a known inductor of heme oxygenase, also induced p31 synthesis as well as heme oxygenase activity in the cells. On two-dimensional gel electrophoresis, p31 induced by DELTA(12)-PGJ2 exhibited an isoelectric point of 5.4, which coincided exactly with that induced by hemin. These results indicate that the p31 induced by DELTA(12)-PGJ2 in porcine aortic endothelial cells is heme oxygenase.

引用

页码：121 / 131

页数：11

共 22 条

[1]

ATSMON J, 1990, CANCER RES, V50, P1879

[2]

CALTABIANO MM, 1986, J BIOL CHEM, V261, P3381

[3] REGULATION OF FERRITIN AND HEME OXYGENASE SYNTHESIS IN RAT FIBROBLASTS BY DIFFERENT FORMS OF IRON [J].