GLASS-CAPILLARY GAS-CHROMATOGRAPHY WITH ELECTRON-CAPTURE DETECTION - SEPARATION OF PROSTAGLANDINS

被引：41

作者：

FITZPATRICK, FA ^{[1
]}

STRINGFELLOW, DA ^{[1
]}

MACLOUF, J ^{[1
]}

RIGAUD, M ^{[1
]}

机构：

[1] CHU DUPUYTREN,BIOCHEM LAB,F-87031 LIMOGES,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 177卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(00)92598-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Glass capillary gqs chromatography of the prostaglandins was performed on a system including an all-glass, solventless injector; thermostable methylphenylpolysiloxane glass capillary columns; and a conventional electron-capture detector fitted with a make-up gas tee. The principal stable metabolites of prostaglandin endoperoxide were separated as perfluorinated derivatives in 35min. Detection limits to equal or exceed those obtained for packed column separations and electron-capture detection. Prostaglandin endoperoxide metabolic profiles from mammalian cell cultures were obtained using this system. These profiling studies are not possible with other chromatographic methods because of inferior resolution and sensitivity. © 1979.

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页码：51 / 60

页数：10

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