PARTIAL CHARACTERIZATION OF PRESUMPTIVE MYOSIN MESSENGER RIBONUCLEIC ACID

被引：97

作者：

HEYWOOD, SM

NWAGWU, M

机构：

[1] Institute of Cellular Biology, University of Connecticut, Storrs, Connecticut

来源：

BIOCHEMISTRY | 1969年 / 8卷 / 09期

关键词：

D O I：

10.1021/bi00837a050

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure is described for obtaining high molecular weight ribonucleic acid from myosin-synthesizing polysomes. Analysis of this ribonucleic acid by 32P labeling and sucrose density gradient centrifugation reveals, in addition to the characteristic ribosomal ribonucleic acid (18 and 28 S), a unique species with a sedimentation coefficient of approximately 26 S. This species of ribonucleic acid is not found to be associated with nonmyosin-synthesizing polysomes or single ribosomes. The 26S ribonucleic acid was judged as presumptive messenger ribonucleic acid for the large 200,000 molecular weight subunit of myosin by the following criteria. When added to a cell-free system, it caused the formation of polysomes; it caused the synthesis of a protein which both precipitated with antimyosin and cochromatographed with myosin on DEAE-cellulose; and it specifically resulted in the synthesis of the large, major component of myosin distinguished by acrylamide gel electrophoresis. © 1969, American Chemical Society. All rights reserved.

引用

页码：3839 / &

共 26 条

[1] STUDIES OF MUSCLE DEVELOPMENT .2. IMMUNOLOGICAL AND ENZYMATIC PROPERTIES AND ACCUMULATION OF CHROMATOGRAPHICALLY HOMOGENEOUS MYOSIN OF LEG MUSCULATURE OF DEVELOPING CHICK [J].