DIAZOTIZED M-AMINOBENZYLOXYMETHYLCELLULOSE AS INSOLUBLE MATRIX FOR AN IMMUNOADSORBENT USED IN PURIFICATION OF ANTIGENS AND ANTIBODIES

The use of diazotized m-aminobenzyloxymethylcellulose as the insoluble matrix for immunoadsorbents capable of purifying rabbit antibovine serum albumin and human C-reactive protein directly from serum has been explored. Based on the quantitative precipitin test and Lowry protein determinations, antibody with a specific activity (milligram of protein precipitable at the equivalence peak per milligram of total protein) of 0.98 has been isolated. In addition, C-reactive protein was recovered from appropriate immunoadsorbent columns, but in a less highly purified form. The yield of antibovine serum albumin per gram of adsorbent was 100 times that obtained for C-reactive protein. © 1969, American Chemical Society. All rights reserved.