DETERMINATION OF ENZYMATIC ACTIVITY IN POLYACRYLAMIDE GELS .I. ENZYMES CATALYZING CONVERSION OF NONREDUCING SUBSTRATES TO REDUCING PRODUCTS

被引：241

作者：

GABRIEL, O

WANG, SF

机构：

[1] Georgetown University, Schools of Medicine and Dentistry, Department of Biochemistry, Washington

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 27卷 / 03期

关键词：

D O I：

10.1016/0003-2697(69)90068-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and sensitive method for the location of enzymes after disc gel electrophoresis has been developed by a modification of the well-known formazan reaction. By means of the same assay, semiquantitative evaluation of the enzymic activity can also be accomplished, permitting measurement of enzymic activity in crude extracts. As has been illustrated by several examples, the procedures can be modified and adapted for the detection and measurement of numerous enzymes. The method is applicable to all enzymes converting nonreducing substrates into products exhibiting reducing properties. In addition, macromolecules susceptible to periodate oxidation can be selectively stained employing the same procedure. © 1969.

引用

页码：545 / &

共 12 条

[1]

CRAMBACH A, 1967, ANAL BIOCHEM, V20, P150

[2] DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS [J].