ISOLATION OF ADENOVIRUS TYPE-5 HOST RANGE DELETION MUTANTS DEFECTIVE FOR TRANSFORMATION OF RAT EMBRYO CELLS

被引：728

作者：

JONES, N ^{[1
]}

SHENK, T ^{[1
]}

机构：

[1] UNIV CONNECTICUT,CTR HLTH SCI,DEPT MICROBIOL,FARMINGTON,CT 06032

来源：

CELL | 1979年 / 17卷 / 03期

关键词：

D O I：

10.1016/0092-8674(79)90275-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of adenovirus type 5 (Ad5) deletion, insertion and substitution mutants, some of which are defective for transformation of rat cells, have been isolated. The mutants were selected as variants which lack the Xba I endonuclease cleavage site at 4 map units on the viral chromosome. The deletions range in size from 150-2300 bp and are located between 1.5 and 10.5 map units. The mutants can be propagated in 293 cells (Ad5-transformed human embryonic kidney cells), but are defective for growth in HeLa or human embryonic kidney cells. No viral DNA synthesis was observed in mutant virus-infected HeLa cells. All but one of the deletion mutants tested were defective for transformation of rat embryo and rat embryo brain cells. © 1979.

引用

页码：683 / 689

页数：7

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