INTERMEDIATE-SIZED FILAMENTS OF HUMAN-ENDOTHELIAL CELLS

被引:219
作者
FRANKE, WW [1 ]
SCHMID, E [1 ]
OSBORN, M [1 ]
WEBER, K [1 ]
机构
[1] MAX PLANCK INST BIOPHYS CHEM,DEPT BIOCHEM,D-3400 GOTTINGEN,FED REP GER
关键词
D O I
10.1083/jcb.81.3.570
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Human endothelial cells prepared from umbilical cords are characterized in parallel by electron microscopy and indirect immunofluorescence microscopy using specific antibodies against different classes of intermediate-sized filaments. The strongly developed, loose bundles of intermediate-sized filaments typically found in these cells are not decorated by antibodies against prekeratin or anitbodies against smooth muscle desmin. They are, however, strongly decorated by antibodies directed against murine 'vimentin,' i.e., the 57,000 mol wt polypeptide which is the major protein of the intermediate-sized filaments predominant in various cells of mesenchymal origin. Cytoskeletal preparations greatly enriched in intermediate-sized filaments show the enrichment of a polypeptide band comigrating with murine vimentin. This shows that the intermediate-sized filaments that are abundant in human endothelial cells are predominantly of the vimentin type and can be demonstrated by their cross-reaction with the vimentin of rodents. These data also strengthen the evidence for several subclasses of intermediate-sized filaments, which can be distinguished by immunological procedures.
引用
收藏
页码:570 / 580
页数:11
相关论文
共 43 条
[1]   DIFFERENCES AMONG 100-A FILAMENT SUBUNITS FROM DIFFERENT CELL-TYPES [J].
BENNETT, GS ;
FELLINI, SA ;
CROOP, JM ;
OTTO, JJ ;
BRYAN, J ;
HOLTZER, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (09) :4364-4368
[2]   LOCALIZATION OF BOVINE BRAIN FILAMENT ANTIBODY ON INTERMEDIATE (100A) FILAMENTS IN GUINEA-PIG VASCULAR ENDOTHELIAL CELLS AND CHICK CARDIAC-MUSCLE CELLS [J].
BLOSE, SH ;
SHELANSKI, ML ;
CHACKO, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (02) :662-665
[3]  
BLOSE SH, 1975, DEV GROWTH DIFFER, V17, P153
[4]   RINGS OF INTERMEDIATE (100 A) FILAMENT BUNDLES IN PERINUCLEAR REGION OF VASCULAR ENDOTHELIAL CELLS - THEIR MOBILIZATION BY COLCEMID AND MITOSIS [J].
BLOSE, SH ;
CHACKO, S .
JOURNAL OF CELL BIOLOGY, 1976, 70 (02) :459-466
[5]   FILAMENTOUS CYTOSKELETON IN VERTEBRATE SMOOTH-MUSCLE FIBERS [J].
COOKE, P .
JOURNAL OF CELL BIOLOGY, 1976, 68 (03) :539-556
[6]   CLASSES OF DISTINGUISHABLE 10 NM CYTOPLASMIC FILAMENTS [J].
DAVISON, PF ;
HONG, BS ;
COOKE, P .
EXPERIMENTAL CELL RESEARCH, 1977, 109 (02) :471-474
[7]   STRUCTURE AND BIOCHEMICAL COMPOSITION OF DESMOSOMES AND TONOFILAMENTS ISOLATED FROM CALF MUZZLE EPIDERMIS [J].
DROCHMANS, P ;
FREUDENSTEIN, C ;
WANSON, JC ;
LAURENT, L ;
KEENAN, TW ;
STADLER, J ;
LELOUP, R ;
FRANKE, WW .
JOURNAL OF CELL BIOLOGY, 1978, 79 (02) :427-443
[8]   SELECTIVE BINDING OF ANTIBODY AGAINST GIZZARD 10-NM FILAMENTS TO DIFFERENT CELL-TYPES IN MYOGENIC CULTURES .1. [J].
FELLINI, SA ;
BENNETT, GS ;
HOLTZER, H .
AMERICAN JOURNAL OF ANATOMY, 1978, 153 (03) :451-457
[9]   RELATIONSHIP OF NUCLEAR MEMBRANES WITH FILAMENTS AND MICROTUBULES [J].
FRANKE, WW .
PROTOPLASMA, 1971, 73 (02) :263-+
[10]  
FRANKE WW, 1978, CYTOBIOLOGIE, V17, P365