TOWARD RULES RELATING ZINC FINGER PROTEIN SEQUENCES AND DNA-BINDING SITE PREFERENCES

被引：204

作者：

DESJARLAIS, JR ^{[1
]}

BERG, JM ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV, SCH MED, DEPT BIOPHYS & BIOPHYS CHEM, BALTIMORE, MD 21205 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 16期

关键词：

TRANSCRIPTION FACTORS; SP1; MOLECULAR RECOGNITION;

D O I：

10.1073/pnas.89.16.7345

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Zinc finger proteins of the Cys2-His2 type consist of tandem arrays of domains, where each domain appears to contact three adjacent base pairs of DNA through three key residues. We have designed and prepared a series of variants of the central zinc ringer within the DNA binding domain of Sp1 by using information from an analysis of a large data base of zinc finger protein sequences. Through systematic variations at two of the three contact positions (underlined), relatively specific recognition of sequences of the form 5'-GGGGN(G or T)GGG-3' has been achieved. These results provide the basis for rules that may develop into a code that will allow the design of zinc ringer proteins with preselected DNA site specificity.

引用

页码：7345 / 7349

页数：5

共 19 条

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