DEVELOPMENT OF A BIOARTIFICIAL LIVER USING ISOLATED HEPATOCYTES

被引:74
作者
DIXIT, V
机构
[1] Department of Medicine, Ucla School of Medicine, Los Angeles, California
关键词
D O I
10.1111/j.1525-1594.1994.tb02219.x
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Severe liver disease is very often life-threatening and dramatically diminishes quality of life. Liver support systems based on detoxification alone have been proved ineffective because they cannot correct biochemical disorders. An effective artificial liver support system should be capable of carrying out the liver's essential processes, such as synthetic and metabolic functions, detoxification, and excretion. It should be capable of sustaining patients with fulminant hepatic failure, preparing patients for liver transplantation when a donor liver is not readily available (i.e., bridge to transplantation), and improving the survival and quality of life for patients for whom transplantation is not a therapeutic option. Recent advances in cell biology, tissue culture techniques, and biotechnology have led the way for the potential use of isolated hepatocytes in treating an array of liver disorders. Isolated hepatocytes may be transplanted to replace liver-specific deficiencies or as an important element of an auxiliary hybrid, bioartificial extracorporeal liver support device, which are important therapeutic applications for treating severe liver disease. Recently, several hepatocyte-based liver support systems have been proposed. Although there is no current consensus on its eventual design configuration, the hollow fiber hepatocyte bioreactor shows the greatest promise. Furthermore, application of tissue engineering technology, based on cell-surface interaction studies proposed by our group and others, has enhanced interest in the development of highly efficient hybrid, bioartificial, liver support devices.
引用
收藏
页码:371 / 384
页数:14
相关论文
共 70 条
[1]  
AEBISCHER P, 1991, EXP NEUROL, V111, P267
[2]   TISSUE REACTION TO SUBCUTANEOUS IMPLANTATION OF A COLLAGEN SPONGE - A HISTOLOGICAL, ULTRASTRUCTURAL, AND IMMUNOLOGICAL STUDY [J].
ANSELME, K ;
BACQUES, C ;
CHARRIERE, G ;
HARTMANN, DJ ;
HERBAGE, D ;
GARRONE, R .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1990, 24 (06) :689-703
[3]   DEVELOPMENT OF BIOARTIFICIAL LIVER - BILIRUBIN CONJUGATION IN GUNN-RATS [J].
ARNAOUT, WS ;
MOSCIONI, AD ;
BARBOUR, RL ;
DEMETRIOU, AA .
JOURNAL OF SURGICAL RESEARCH, 1990, 48 (04) :379-382
[4]  
BERRY MN, 1991, LABORATORY TECHNIQUE, P395
[5]   LIVER PRESERVATION - THE PAST AND THE FUTURE [J].
BLANKENSTEIJN, JD ;
TERPSTRA, OT .
HEPATOLOGY, 1991, 13 (06) :1235-1250
[6]   HEPATOCYTES IMMOBILIZED BY MICROENCAPSULATION IN ARTIFICIAL CELLS - EFFECTS ON HYPERBILIRUBINEMIA IN GUNN-RATS [J].
BRUNI, S ;
CHANG, TMS .
BIOMATERIALS ARTIFICIAL CELLS AND ARTIFICIAL ORGANS, 1989, 17 (04) :403-411
[7]   DEVELOPMENT AND EVALUATION OF A SYSTEM OF MICROENCAPSULATION OF PRIMARY RAT HEPATOCYTES [J].
CAI, ZH ;
SHI, ZQ ;
SHERMAN, M ;
SUN, AM .
HEPATOLOGY, 1989, 10 (05) :855-860
[8]   ARTIFICIAL CELLS IN MEDICINE AND BIOTECHNOLOGY [J].
CHANG, TMS .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1984, 10 :5-24
[9]   SEMIPERMEABLE MICROCAPSULES [J].
CHANG, TMS .
SCIENCE, 1964, 146 (364) :524-&
[10]  
CHANG TMS, 1984, LIFE SUPPORT SYST, P461