PHALLOIDIN REDUCES THE RELEASE OF INORGANIC-PHOSPHATE DURING ACTIN POLYMERIZATION

被引：36

作者：

DANCKER, P

HESS, L

机构：

[1] Institut für Zoologie, Technische Hochschule Darmstadt, Darmstadt

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1035卷 / 02期

关键词：

(Rabbit skeletal muscle); Actin polymerization; ATP hydrolysis; Phalloidin; Phosphate;

D O I：

10.1016/0304-4165(90)90116-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phalloidin, an actin-filament stabilizing peptide from Amanita phalloides, did not inhibit ATP hydrolysis during actin polymerization but strongly retarded the release of the hydrolysis product Pi. Thus, the lifetime of the intermediate F-actin-ADP-P1 is significantly increased by phalloidin. The results suggest a close correlation between filament stability and F-actin-ADP-Pi intermediates. © 1990.

引用

收藏

页码：197 / 200

页数：4

相关论文

共 11 条

[1] MEASUREMENT OF PI DISSOCIATION FROM ACTIN-FILAMENTS FOLLOWING ATP HYDROLYSIS USING A LINKED ENZYME ASSAY [J].

CARLIER, MF .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 143 (03) :1069-1075

[2]

CARLIER MF, 1989, INT REV CYTOL, V115, P139

[3] THE HYDROLYSIS OF ATP THAT ACCOMPANIES ACTIN POLYMERIZATION IS ESSENTIALLY IRREVERSIBLE [J].

CARLIER, MF ;

PANTALONI, D ;

EVANS, JA ;

LAMBOOY, PK ;

KORN, ED ;

WEBB, MR .

FEBS LETTERS, 1988, 235 (1-2) :211-214

[4] DIRECT EVIDENCE FOR ADP-PI-F-ACTIN AS THE MAJOR INTERMEDIATE IN ATP-ACTIN POLYMERIZATION - RATE OF DISSOCIATION OF PI FROM ACTIN-FILAMENTS [J].

CARLIER, MF ;

PANTALONI, D .

BIOCHEMISTRY, 1986, 25 (24) :7789-7792

[5] INTERACTION OF ACTIN WITH PHALLOIDIN - POLYMERIZATION AND STABILIZATION OF F-ACTIN [J].

DANCKER, P ;

LOW, I ;

HASSELBACH, W ;

WIELAND, T .

BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 400 (02) :407-404

[6] STUDIES ON THE POLYMERIZATION OF ACTIN - A RAPID METHOD FOR THE SEPARATION OF THE MONOMERIC FROM THE POLYMERIC SPECIES [J].

GRAZI, E ;

MAGRI, E .

FEBS LETTERS, 1981, 123 (02) :193-194

[7] THE INITIAL PHOSPHATE BURST IN ATP HYDROLYSIS BY MYOSIN AND SUBFRAGMENT-1 AS STUDIED BY A MODIFIED MALACHITE GREEN METHOD FOR DETERMINATION OF INORGANIC-PHOSPHATE [J].

KODAMA, T ;

FUKUI, K ;

KOMETANI, K .

JOURNAL OF BIOCHEMISTRY, 1986, 99 (05) :1465-1472

[8] POLYMORPHISM OF F-ACTIN .2. ATPASE ACTIVITY AT ACID PH [J].

KURODA, M ;

MARUYAMA, K .

JOURNAL OF BIOCHEMISTRY, 1972, 71 (01) :39-&

[9]

PARDEE JD, 1982, METHOD ENZYMOL, V85, P164

[10] ADENOSINETRIPHOSPHATE THE FUNCTIONAL GROUP OF ACTIN [J].

STRAUB, FB ;

FEUER, G .

BIOCHIMICA ET BIOPHYSICA ACTA, 1950, 4 (04) :455-470