AN EXAMINATION OF CONDITIONS FOR CLEAVAGE OF POLYPEPTIDE CHAINS WITH CYANOGEN BROMIDE - APPLICATION TO CATALASE

被引：280

作者：

SCHROEDER, WA

SHELTON, JB

SHELTON, JR

机构：

[1] Division of Chemistry and Chemical Engineering, 1 1 Contribution No. 3779 from the Division of Chemistry, Chemical Engineering. California Institute of Technology, Pasadena

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 130卷 / 1-2期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-9861(69)90069-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The poor cleavage of a -Met-Thr- bond in catalase by cyanogen bromide has been improved by modifying the conditions of the reaction. More complete cleavage occurs in 70% trifluoroacetic acid than in 70% formic acid. The much slower rate of reaction in trifluoroacetic acid has been enhanced by increasing the concentration of the reactants. © 1969.

引用

页码：551 / +

页数：1

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