HIGH-FIDELITY GENE TARGETING IN EMBRYONIC STEM-CELLS BY USING SEQUENCE REPLACEMENT VECTORS

被引：105

作者：

THOMAS, KR ^{[1
]}

DENG, CX ^{[1
]}

CAPECCHI, MR ^{[1
]}

机构：

[1] UNIV UTAH, MED CTR,ECCLES INST HUMAN GENET,HHMI RES LABS, SUITE 5400, SALT LAKE CITY, UT 84112 USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1992年 / 12卷 / 07期

关键词：

D O I：

10.1128/MCB.12.7.2919

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mutations were targeted to the Hprt locus in murine embryonic stem cells by using sequence replacement vectors. When the vector was designed such that the mutated sequences were flanked on both sides by several kilobases of DNA homologous to the target locus, replacement of chromosomal sequences with the exogenous DNA occurred with precision. If, on the other hand, the target-homologous DNA on one arm of the vector was reduced to below 1 kb in length, the fidelity of recombination was diminished.

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页码：2919 / 2923

页数：5

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