MUTATIONS OF THE ADENYLYL CYCLASE GENE THAT BLOCK RAS FUNCTION IN SACCHAROMYCES-CEREVISIAE

被引：104

作者：

FIELD, J

XU, HP

MICHAELI, T

BALLESTER, R

SASS, P

WIGLER, M

COLICELLI, J

机构：

[1] Cold Spring Harbor Laboratory, Cold Spring Harbor

[2] Lederle Laboratory, Pearl River

来源：

SCIENCE | 1990年 / 247卷 / 4941期

关键词：

D O I：

10.1126/science.2405488

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The interaction between RAS proteins and adenylyl cyclase was studied by using dominant interfering mutations of adenylyl cyclase from the yeast Saccharomyces cerevisiae. RAS proteins activate adenylyl cyclase in this organism. A plasmid expressing a catalytically inactive adenylyl cyclase was found to interfere dominantly with this activation. The interfering region mapped to the leucine-rich repeat region of adenylyl cyclase, which is homologous to domains present in several other proteins and is thought to participate in protein-protein interactions.

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页码：464 / 467

页数：4

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