DISTRIBUTION AND PROPERTIES OF CDP-DIGLYCERIDE - INOSITOL TRANSFERASE FROM BRAIN

被引：120

作者：

BENJAMINS, JA

AGRANOFF, BW

机构：

[1] Department of Biochemistry, Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan

来源：

JOURNAL OF NEUROCHEMISTRY | 1969年 / 16卷 / 04期

关键词：

D O I：

10.1111/j.1471-4159.1969.tb06850.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CDP‐diglyceride is converted to phosphatidyl inositol by several particulate subcellular fractions of guinea pig brain, with highest specific activity in the microsomal fraction. Optimal conditions with respect to pH, metal ion concentration, and substrate concentrations have been determined. The reaction was stimulated by the addition of bovine serum albumin and by Tween 80. Of several dl‐CDP‐diglycerides synthesized and used as substrates in a spectrophoto‐metric assay for the enzyme, dl‐CDP‐didecanoin was the most active. The enzyme showed a high selectivity for myo‐inositol. Of a number of compounds tested, only scyllo‐inosose and epi‐inosose served as substrates. Three inositol isomers and three myo‐inositol monophosphates inhibited the reaction slightly. The most potent inhibitor found was galactinol, a myo‐inositol galactoside. Copyright © 1969, Wiley Blackwell. All rights reserved

引用

页码：513 / +

页数：1

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