MULTIPLE FLUORESCENCE INSITU HYBRIDIZATION

被引：212

作者：

NEDERLOF, PM

VANDERFLIER, S

WIEGANT, J

RAAP, AK

TANKE, HJ

PLOEM, JS

VANDERPLOEG, M

机构：

[1] Sylvius Laboratories, Department of Cytochemistry and Cytometry, University of Leiden, Leiden, 2333 AL

来源：

CYTOMETRY | 1990年 / 11卷 / 01期

关键词：

Chromosomal aberrations; fluorescence ratio imaging; oncology; prenatal diagnosis;

D O I：

10.1002/cyto.990110115

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for multiple fluorescence in situ hybridization is described allowing the simultaneous detection of more than three target sequences with only three fluorescent dyes (FITC, TRITC, AMCA), respectively emitting in the green, red, and blue. This procedure is based on the labeling of (DNA) probes with more than one hapten and visualisation in multiple colors. The possibility to detect multiple targets simultaneously is important for prenatal diagnosis and the detection of numerical and/or structural chromosome aberrations in tumor diagnosis. It may form the basis for an in situ hybridization based chromosome banding technique. Copyright © 1990 Wiley‐Liss, Inc.

引用

收藏

页码：126 / 131

页数：6

相关论文

共 19 条

[1] FLUORESCENCE DIGITAL IMAGING MICROSCOPY IN CELL BIOLOGY [J].

ARNDTJOVIN, DJ ;

ROBERTNICOUD, M ;

KAUFMAN, SJ ;

JOVIN, TM .

SCIENCE, 1985, 230 (4723) :247-256

[2] RAPID INTERPHASE AND METAPHASE ASSESSMENT OF SPECIFIC CHROMOSOMAL CHANGES IN NEUROECTODERMAL TUMOR-CELLS BY INSITU HYBRIDIZATION WITH CHEMICALLY MODIFIED DNA PROBES [J].

CREMER, T ;

TESIN, D ;

HOPMAN, AHN ;

MANUELIDIS, L .

EXPERIMENTAL CELL RESEARCH, 1988, 176 (02) :199-220

[3] DETECTION OF CHROMOSOME-ABERRATIONS IN METAPHASE AND INTERPHASE TUMOR-CELLS BY INSITU HYBRIDIZATION USING CHROMOSOME-SPECIFIC LIBRARY PROBES [J].

CREMER, T ;

LICHTER, P ;

BORDEN, J ;

WARD, DC ;

MANUELIDIS, L .

HUMAN GENETICS, 1988, 80 (03) :235-246

[4]

DEVILEE P, 1988, CANCER RES, V48, P5825

[5] 2 SUBSETS OF HUMAN ALPHOID REPETITIVE DNA SHOW DISTINCT PREFERENTIAL LOCALIZATION IN THE PERICENTRIC REGIONS OF CHROMOSOME-13, CHROMOSOME-18, AND CHROMOSOME-21 [J].

DEVILEE, P ;

CREMER, T ;

SLAGBOOM, P ;

BAKKER, E ;

SCHOLL, HP ;

HAGER, HD ;

STEVENSON, AFG ;

CORNELISSE, CJ ;

PEARSON, PL .

CYTOGENETICS AND CELL GENETICS, 1986, 41 (04) :193-201

[6] CYANINE DYE LABELING REAGENTS FOR SULFHYDRYL-GROUPS [J].

ERNST, LA ;

GUPTA, RK ;

MUJUMDAR, RB ;

WAGGONER, AS .

CYTOMETRY, 1989, 10 (01) :3-10

[7] ORGANIZATION OF A REPETITIVE HUMAN 1.8 KB KPNI SEQUENCE LOCALIZED IN THE HETEROCHROMATIN OF CHROMOSOME-15 [J].

HIGGINS, MJ ;

WANG, H ;

SHTROMAS, I ;

HALIOTIS, T ;

RODER, JC ;

HOLDEN, JJA ;

WHITE, BN .

CHROMOSOMA, 1985, 93 (01) :77-86

[8] INSITU HYBRIDIZATION AS A TOOL TO STUDY NUMERICAL CHROMOSOME-ABERRATIONS IN SOLID BLADDER-TUMORS [J].

HOPMAN, AHN ;

RAMAEKERS, FCS ;

RAAP, AK ;

BECK, JLM ;

DEVILEE, P ;

VANDERPLOEG, M ;

VOOIJS, GP .

HISTOCHEMISTRY, 1988, 89 (04) :307-316

[9] IMPROVED FLOW CYTOMETRIC ANALYSIS OF LEUKOCYTE SUBSETS - SIMULTANEOUS IDENTIFICATION OF 5 CELL SUBSETS USING 2-COLOR IMMUNOFLUORESCENCE [J].

HORAN, PK ;

SLEZAK, SE ;

POSTE, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (21) :8361-8365

[10] AMINOMETHYL COUMARIN ACETIC-ACID - A NEW FLUORESCENT LABELING AGENT FOR PROTEINS [J].

KHALFAN, H ;

ABUKNESHA, R ;

RANDWEAVER, M ;

PRICE, RG ;

ROBINSON, D .

HISTOCHEMICAL JOURNAL, 1986, 18 (09) :497-&