ANALYSIS OF XENOPUS-LAEVIS GLOBINS DURING DEVELOPMENT AND ERYTHROID CELL MATURATION AND THE CONSTRUCTION OF RECOMBINANT PLASMIDS CONTAINING SEQUENCES DERIVED FROM ADULT GLOBIN MESSENGER-RNA

被引：47

作者：

HENTSCHEL, CC ^{[1
]}

KAY, RM ^{[1
]}

WILLIAMS, JG ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND, MILL HILL LABS, LONDON NW7 1AD, ENGLAND

来源：

DEVELOPMENTAL BIOLOGY | 1979年 / 72卷 / 02期

关键词：

D O I：

10.1016/0012-1606(79)90124-6

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Developmental changes in the globin polypeptide composition of Xenopus laevis erythrocytes were examined by acid-urea polyacrylamide gel electrophoresis and a major switch from tadpole to adult globins was detected after metamorphic climax. The coding capacity of mRNA derived from mature adult erythroid cells was examined by cell-free translation and the products were shown to coelectrophorese with the globins of the adult erythroid cells. We describe the molecular cloning of sequences present in this mRNA and the characterisation of clones derived from one of the major globins and one clone derived from a minor adult globin mRNA. © 1979.

引用

页码：350 / 363

页数：14

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