ADP-RIBOSYLATION OF THE RHO/RAC PROTEINS INDUCES GROWTH-INHIBITION, NEURITE OUTGROWTH AND ACETYLCHOLINE ESTERASE IN CULTURED PC-12 CELLS

被引：119

作者：

NISHIKI, T

NARUMIYA, S

MORII, N

YAMAMOTO, M

FUJIWARA, M

KAMATA, Y

SAKAGUCHI, G

KOZAKI, S

机构：

[1] KYOTO UNIV,FAC MED,DEPT PHARMACOL,SAKYO KU,KYOTO 606,JAPAN

[2] UNIV OSAKA PREFECTURE,COLL AGR,DEPT VET SCI,SAKAI,OSAKA 591,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 167卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91760-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Botulinum ADP-ribosyltransferase C3 (C3 exoenzyme) was purified to homogeneity and added to cultured rat pheochromocytoma PC-12 cells. Incubation with this exoenzyme caused inhibition of cell growth and induced neurites as well as acetylcholine esterase in these cells. These changes were dependent on the amount of the enzyme added to the culture, which correlated with the in situ ADP-ribosylation of the rho rac proteins in the cells. Preincubation with a specific anti-C3 exoenzyme monoclonal antibody inhibited both the ADP-ribosyl-transferase activity and the neurite-inducing activity of the enzyme preparation. These results suggest that C3 exoenzyme affected the cellular function of the rho rac proteins by ADP-ribosylation to induce these changes in the cells. © 1990.

引用

页码：265 / 272

页数：8

共 22 条

[1] CLOSTRIDIUM-BOTULINUM TYPE-C PRODUCES A NOVEL ADP-RIBOSYLTRANSFERASE DISTINCT FROM BOTULINUM-C2 TOXIN [J].