A MODIFIED COLORIMETRIC MICRO METHOD FOR LONG-CHAIN FATTY ACIDS AND ITS APPLICATION FOR ASSAY OF LIPOLYTIC ENZYMES

被引：76

作者：

MAHADEVAN, S

DILLARD, CJ

TAPPEL, AL

机构：

[1] Department of Food Science and Technology, University of California, Davis

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 27卷 / 03期

关键词：

D O I：

10.1016/0003-2697(69)90052-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A modification of the Duncombe method for measurement of free fatty acids is presented. Substitution of diphenylcarbohydrazide for diethyldithiocarbamate color complexing agent increases the sensitivity of the method 4- to 6-fold. The absorbancy of the color produced is measured at 540 mμ. Applications of the method for the assay of pancreatic lipase, and rat liver lysosomal lipase and phospholipase are described. © 1969.

引用

页码：387 / +

页数：1

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