TISSUE DISTRIBUTION AND CHARACTERIZATION OF SUCROSE SYNTHASE ISOZYMES IN SUGARCANE

被引：41

作者：

BUCZYNSKI, SR ^{[1
]}

THOM, M ^{[1
]}

CHOUREY, P ^{[1
]}

MARETZKI, A ^{[1
]}

机构：

[1] UNIV FLORIDA, USDA ARS, GAINESVILLE, FL 32611 USA

来源：

JOURNAL OF PLANT PHYSIOLOGY | 1993年 / 142卷 / 06期

关键词：

MONCLONAL ANTIBODIES; SACCHARUM; SUCROSE SYNTHASE ISOZYMES;

D O I：

10.1016/S0176-1617(11)80895-3

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The two isozymic forms of sucrose synthase in sugarcane have been investigated to determine their respective tissue distribution in the plant and differences in their biochemical characteristics. Separation of the isozymic forms was aided by the application of immuno-separation for one form (SS2), whereas the other was purified from older internode tissue in which only one form is present. All other tissue examined contained both forms of this enzyme. The two isoforms of sucrose synthase from sugarcane resemble those found in maize sufficiently to enable the use of maize monoclonal antibodies for their detection. The two sucrose synthase isoforms also resemble each other very closely, as shown by their kinetic and pH characteristics.

引用

页码：641 / 646

页数：6

共 23 条

[1] SUCROSE SYNTHASE IN DEVELOPING MAIZE LEAVES - REGULATION OF ACTIVITY BY PROTEIN LEVEL DURING THE IMPORT TO EXPORT TRANSITION [J].