REPLICATION OF THE COLICIN-E1 PLASMID IN EXTRACTS OF ESCHERICHIA-COLI - UNCOUPLING OF LEADING STRAND FROM LAGGING STRAND SYNTHESIS

被引:38
作者
STAUDENBAUER, WL
SCHERZINGER, E
LANKA, E
机构
[1] Abteilung Schuster, Max-Planck-Institut für Molekulare Genetik, Berlin 33
来源
MOLECULAR AND GENERAL GENETICS | 1979年 / 177卷 / 01期
关键词
D O I
10.1007/BF00267260
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The replication of the ColE1 plasmid was studied in extracts from E. coli dnaG mutants. It was found that the synthesis of the complementary strands of ColE1 DNA can be carried out in these extracts in two consecutive steps: (1) synthesis of the leading L strand independent of the dnaG function, and (2) synthesis of the lagging H strand depending upon addition of wild-type dnaG protein. In contrast to L strand synthesis, the latter reaction is insensitive to rifampicin and novobiocin. Both synthetic pathways are however blocked by antiserum directed against dnaB protein. This indicates an additional role of the dnaB protein in duplex DNA replication besides assisting the dnaG protein in the priming of lagging strand synthesis. The T7 gene-4 protein acting in conjunction with T7 DNA polymerase can substitute for both the function of the dnaB and dnaG protein. It is concluded that plasmid replication proceeds by a semi-discontinuous mechanism. © 1979 Springer-Verlag.
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页码:113 / 120
页数:8
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