MOLECULAR-CLONING OF AN APOLIPOPROTEIN-B MESSENGER-RNA EDITING PROTEIN

被引：511

作者：

TENG, BB ^{[1
]}

BURANT, CF ^{[1
]}

DAVIDSON, NO ^{[1
]}

机构：

[1] UNIV CHICAGO,DEPT MED,MC 4076,CHICAGO,IL 60637

来源：

SCIENCE | 1993年 / 260卷 / 5115期

关键词：

D O I：

10.1126/science.8511591

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mammalian apolipoprotein B (apo B) exists in two forms, each the product of a single gene. The shorter form, apo B48, arises by posttranscriptional RNA editing whereby cytidine deamination produces a UAA termination codon. A full-length complementary DNA clone encoding an apo B messenger RNA editing protein (REPR) was isolated from rat small intestine. The 229-residue protein contains consensus phosphorylation sites and leucine zipper domains. HepG2 cell extracts acquire editing activity when mixed with REPR from oocyte extracts. REPR is essential for apo B messenger RNA editing, and the isolation and characterization of REPR may lead to the identification of other eukaryotic RNA editing proteins.

引用

页码：1816 / 1819

页数：4

共 29 条

[1] APOLIPOPROTEIN-B MESSENGER-RNA SEQUENCES 3' OF THE EDITING SITE ARE NECESSARY AND SUFFICIENT FOR EDITING AND EDITOSOME ASSEMBLY [J].