MONITORING OF HUMAN CYTOMEGALOVIRUS INFECTIONS AND GANCICLOVIR TREATMENT IN HEART-TRANSPLANT RECIPIENTS BY DETERMINATION OF VIREMIA, ANTIGENEMIA, AND DNAEMIA

被引：284

作者：

GERNA, G ^{[1
]}

ZIPETO, D ^{[1
]}

PAREA, M ^{[1
]}

REVELLO, MG ^{[1
]}

SILINI, E ^{[1
]}

PERCIVALLE, E ^{[1
]}

ZAVATTONI, M ^{[1
]}

GROSSI, P ^{[1
]}

MILANESI, G ^{[1
]}

机构：

[1] CNR,IST GENET BIOCHIM & EVOLUZIONIST,PAVIA,ITALY

来源：

JOURNAL OF INFECTIOUS DISEASES | 1991年 / 164卷 / 03期

关键词：

D O I：

10.1093/infdis/164.3.488

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Fourteen heart transplant recipients were monitored for human cytomegalovirus (HCMV) infection based on determination of antigenemia, viremia, and DNAemia (by polymerase chain reaction [PCR]) in peripheral blood polymorphonuclear leukocytes (PMNL). Three patients had symptomatic primary, 10 had recurrent (3 asymptomatic), and 1 (seronegative) had no HCMV infection. Severe clinical symptoms appeared when levels of viremia/antigenemia were > 50 infected PMNL/2 x 10(5) cells examined. Of 200 blood samples examined, 93 (46.5%) were positive for viremia/antigenemia and DNAemia, whereas 48 (24.0%) were positive for DNAemia only; 59 (29.5%) were negative in all assays. Follow-up of HCMV infections in heart transplant recipients showed that PCR can detect viral appearance in blood 7-10 days earlier than assays for antigenemia/viremia. On the other hand, viral disappearance from blood, as assessed by PCR, occurred weeks or months later than revealed by other assays. Detection of virus by PCR only was never associated with overt HCMV-related clinical symptoms. Of the 8 symptomatic patients treated with ganciclovir, 2 became PCR-negative at the end of treatment and 1 cleared virus from blood in the following weeks, whereas 5 showed persistent or recurrent infection.

引用

页码：488 / 498

页数：11

共 38 条

[1] THE STRUCTURE OF THE MAJOR IMMEDIATE EARLY GENE OF HUMAN CYTOMEGALO-VIRUS STRAIN AD169 [J].