ROLE OF ISOPRENYLATION IN INTRACELLULAR PH REGULATION OF GRANULOCYTES

被引：8

作者：

ARELLANO, R ^{[1
]}

GRINSTEIN, S ^{[1
]}

机构：

[1] HOSP SICK CHILDREN,RES INST,DIV CELL BIOL,555 UNIV AVE,TORONTO M5G 1X8,ONTARIO,CANADA

来源：

FEBS LETTERS | 1993年 / 332卷 / 03期

关键词：

NA+/H+ EXCHANGE; ANTIPORT; NADPH OXIDASE; LOVASTATIN; HL-60; CELL;

D O I：

10.1016/0014-5793(93)80642-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lovastatin was used to study the role of isoprenylated GTP-binding proteins in the regulation of intracellular pH in granulocytic HL-60 cells. The cytosolic acidification that accompanies stimulation by chemoattractants was completely eliminated by lovastatin. The subsequent activation of Na+/H+ exchange was partially inhibited by the isoprenylation antagonist. In contrast, the osmotic activation of the Na+/H+ antiport was unaffected. The data indicate that the osmotically- and receptor-induced activation of the antiport utilize divergent signalling pathways. The results also provide evidence supporting the notion that cytosolic acidification by chemoattractants results from accumulation of metabolic acid generated during the respiratory burst.

引用

页码：247 / 250

页数：4

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