RECOVERY OF FUNCTIONAL PROTEINS FROM SODIUM DODECYL SULFATE-POLYACRYLAMIDE GELS

被引：51

作者：

HANAOKA, F ^{[1
]}

SHAW, JL ^{[1
]}

MUELLER, GC ^{[1
]}

机构：

[1] UNIV WISCONSIN,MCARDLE LAB CANC RES,MADISON,WI 53706

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 99卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(79)90059-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple method and apparatus are described for the efficient recovery of proteins from sodium dodecyl sulfate-polyacrylamide gel systems after electrophoretic resolution. This procedure provides for high yields of proteins which are free of sodium dodecyl sulfate and in certain cases, exhibit significant levels of biological activity. © 1979.

引用

页码：170 / 174

页数：5

共 14 条

[1]

HEINS JN, 1966, PROCEDURES NUCLEIC A, P79

[2] 2 METHODS FOR ELECTROPHORETIC ELUTION OF PROTEINS FROM POLYACRYLAMIDE GELS [J].

KARSNAS, P ;

ROOS, P .

ANALYTICAL BIOCHEMISTRY, 1977, 77 (01) :168-175

[3] ISOLATION OF AN ELECTROPHORETICALLY HOMOGENEOUS NONHISTONE NUCLEOLAR PROTEIN [J].

KNECHT, ME ;

BUSCH, H .

LIFE SCIENCES PT-2 BIOCHEMISTRY GENERAL AND MOLECULAR BIOLOGY, 1971, 10 (22) :1297-&

[4] CRYSTALLINE DESOXYRIBONUCLEASE .1. ISOLATION AND GENERAL PROPERTIES - SPECTROPHOTOMETRIC METHOD FOR THE MEASUREMENT OF DESOXYRIBONUCLEASE ACTIVITY [J].

KUNITZ, M .

JOURNAL OF GENERAL PHYSIOLOGY, 1950, 33 (04) :349-362

[5] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[6] RAPID AND EFFECTIVE REMOVAL OF SODIUM DODECYL SULFATE FROM PROTEINS [J].

LENARD, J .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1971, 45 (03) :662-&

[7]

LIAO TH, 1975, J BIOL CHEM, V250, P3831

[8]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[9] DISSOCIATION AND RECONSTITUTION OF DNA REPLICASE SYSTEM OF HELA-CELL NUCLEI [J].

SEKI, S ;

MUELLER, GC .

BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 435 (03) :236-250

[10] REMOVAL OF SODIUM DODECYL-SULFATE FROM PROTEINS [J].

TUSZYNSKI, GP ;

WARREN, L .

ANALYTICAL BIOCHEMISTRY, 1975, 67 (01) :55-65

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