BROMODEOXYURIDINE MUTAGENESIS IN MAMMALIAN-CELLS - MUTAGENESIS IS INDEPENDENT OF AMOUNT OF BROMOURACIL IN DNA

被引：82

作者：

KAUFMAN, ER ^{[1
]}

DAVIDSON, RL ^{[1
]}

机构：

[1] HARVARD UNIV,CHILDRENS HOSP,SCH MED,DEPT MICROBIOL & MOLEC GENET,BOSTON,MA 02115

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1978年 / 75卷 / 10期

关键词：

D O I：

10.1073/pnas.75.10.4982

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Studies were undertaken to determine how a line of mutant Syrian hamster melanoma cells (HAB-2E) that displays unlimited growth potential when all of the thymine residues in nuclear DNA are replaced by bromouracil (BrUra) could avoid the deleterious effects of bromodeoxyuridine (BrdUrd) mutagenicity. It was found that BrdUrd could be mutagenic to these cells. However, there was a nonlinear relationship between mutagenicity and the amount of BrUra in the DNA of the HAB-2E cells. With these cells, mutagenicity apparently is determined by the concentration of BrdUrd to which the cells are exposed rather than the amount of BrUra in DNA. These results were obtained with both the induction of ouabain resistance and thioguanine resistance as markers for mutagenesis. The dependence of BrdUrd mutagenicity on BrdUrd concentration was also observed for the parental melanoma cells.

引用

页码：4982 / 4986

页数：5

共 20 条

[1] MUTAGENIC MECHANISM OF 5-BROMODEOXYURIDINE IN CHINESE-HAMSTER CELLS [J].

AEBERSOLD, PM .

MUTATION RESEARCH, 1976, 36 (03) :357-362

[2] OUABAIN-RESISTANT MUTANTS OF MOUSE AND HAMSTER CELLS IN CULTURE [J].

BAKER, RM ;

BRUNETTE, DM ;

MANKOVITZ, R ;

THOMPSON, LH ;

WHITMORE, GF ;

SIMINOVITCH, L ;

TILL, JE .

CELL, 1974, 1 (01) :9-21

[3] TOTAL SUBSTITUTION OF BROMODEOXYURIDINE FOR THYMIDINE IN DNA OF A BROMODEOXYURIDINE-DEPENDENT CELL LINE [J].

BICK, MD ;

DAVIDSON, RL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (05) :2082-2086

[4] NUCLEOTIDE ANALYSIS OF DNA AND RNA IN CELLS WITH THYMIDINE TOTALLY REPLACED BY BROMODEOXYURIDINE [J].

BICK, MD ;

DAVIDSON, RL .

SOMATIC CELL GENETICS, 1976, 2 (01) :63-76

[5]

DAVIDSON RL, 1974, P NATL ACAD SCI USA, V71, P3338, DOI 10.1073/pnas.71.9.3338

[6] BROMODEOXYURIDINE DEPENDENCE - NEW MUTATION IN MAMMALIAN-CELLS [J].

DAVIDSON, RL ;

BICK, MD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (01) :138-142

[7] DEOXYCYTIDINE REVERSES SUPPRESSION OF PIGMENTATION CAUSED BY 5-BRDURD WITHOUT CHANGING AMOUNT OF 5-BRDURD IN DNA [J].

DAVIDSON, RL ;

KAUFMAN, ER .

CELL, 1977, 12 (04) :923-929

[8]

DAVIDSON RL, 1965, NATURE, V206, P1170

[9] SPECIFIC MUTAGENIC EFFECT OF BASE ANALOGUES ON PHAGE-T4 [J].

FREESE, E .

JOURNAL OF MOLECULAR BIOLOGY, 1959, 1 (02) :87-105

[10] EFFECTS OF THYMIDINE ANALOGS ON SYRIAN-HAMSTER MELANOMA CELLS - PHENOTYPES ARISING FROM SELECTION FOR ANALOG RESISTANCE [J].

KAUFMAN, ER ;

DAVIDSON, RL .

SOMATIC CELL GENETICS, 1977, 3 (06) :649-661

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