CONSTRUCTION OF PHYSICAL AND GENETIC MAPS OF CHLAMYDIA-TRACHOMATIS SEROVAR-L2 BY PULSED-FIELD GEL-ELECTROPHORESIS

被引：43

作者：

BIRKELUND, S

STEPHENS, RS

机构：

[1] AARHUS UNIV,INST MED MICROBIOL,DK-8000 AARHUS,DENMARK

[2] UNIV CALIF SAN FRANCISCO,DEPT LAB MED,SAN FRANCISCO,CA 94143

来源：

JOURNAL OF BACTERIOLOGY | 1992年 / 174卷 / 09期

关键词：

D O I：

10.1128/JB.174.9.2742-2747.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We constructed the physical map of Chlamydia trachomatis serovar L2 by using three restriction endonucleases, NotI (GC\GGCCGC), SgrAI (C(A/G)\CCGG(T/G)G), and Sse83871 (CCTGCA\GG), and we analyzed the fragments by pulsed-field gel electrophoresis. A total of 25 restriction endonuclease sites and 13 genes and/or operons were located on the map. The genome size was determined to be 1,045 kb. Neither highly transcribed chiamydia genes nor developmental cycle-specific genes were clustered on the genome.

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页码：2742 / 2747

页数：6

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