HIGHLY EFFECTIVE DNA IN STIMULATING POLY-(ADP-RIBOSE) POLYMERASE REACTION

被引：23

作者：

HASHIDA, T

OHGUSHI, H

YOSHIHARA, K

机构：

[1] Department of Biochemistry, Nara Medical University, Kashihara, Nara

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 88卷 / 01期

关键词：

D O I：

10.1016/0006-291X(79)91730-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

During the purification of bovine thymus poly(ADP-ribose) polymerase, a fraction was obtained, which is 20 times as effective in activating the enzyme as the bulk of calf thymus DNA. The method for the isolation and purification of this active fraction is described. The results obtained by chemical analysis, UV-spectrophotometry, and ultracentrifugation of the active fraction indicate that the active substance is double stranded DNA. We suggest that the high enzyme-activating ability of the DNA is due to a DNA sequence(s), concentrated on the DNA, with a high enzyme-binding affinity. © 1979.

引用

页码：305 / 311

页数：7

共 15 条

[1] METHODS FOR CHARACTERIZATION OF NUCLEIC ACIDS BY BASE COMPOSITION [J].