MAPPING THE HUMAN AMYLASE GENE-CLUSTER ON THE PROXIMAL SHORT ARM OF CHROMOSOME-1 USING A HIGHLY INFORMATIVE (CA)N REPEAT

被引：35

作者：

DRACOPOLI, NC

MEISLER, MH

机构：

[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

[2] UNIV MICHIGAN,DEPT HUMAN GENET,ANN ARBOR,MI 48109

来源：

GENOMICS | 1990年 / 7卷 / 01期

关键词：

D O I：

10.1016/0888-7543(90)90523-W

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The human amylase gene cluster includes a (CA)n repeat sequence immediately upstream of the γ-actin pseudogene associated with the AMY2B gene. Analysis of this (CA)n repeat by PCR amplification of genomic DNA from the 40 families of the Centre d'Etude du Polymorphisme Humain (CEPH) reference panel revealed extensive polymorphism. A total of six alleles with (CA)n lengths of 16-21 repeats were found. The average heterozygosity for this polymorphism was 0.70. Multipoint linkage analysis showed that the amylase gene cluster is located distal to the nerve growth factor β-subunit gene (NGFB) and is within 1 cM of the anonymous locus D1S10. The amylase (CA)n repeat provides a convenient marker for both the physical and the genetic maps of human chromosome 1p. © 1990.

引用

页码：97 / 102

页数：6

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