DETECTION OF N-G,N-G-DIMETHYLARGININE DIMETHYLAMINOHYDROLASE IN HUMAN TISSUES USING A MONOCLONAL-ANTIBODY

被引：143

作者：

KIMOTO, M ^{[1
]}

WHITLEY, GS ^{[1
]}

TSUJI, H ^{[1
]}

OGAWA, T ^{[1
]}

机构：

[1] ST GEORGE HOSP,SCH MED,DEPT CELLULAR & MOLEC SCI,LONDON SW17 0RE,ENGLAND

来源：

JOURNAL OF BIOCHEMISTRY | 1995年 / 117卷 / 02期

关键词：

N-G; N-G-DIMETHYLARGININE DIMETHYLAMINOHYDROLASE; NITRIC OXIDE GENERATION; HUMAN TISSUES;

D O I：

10.1093/jb/117.2.237

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two endogenous inhibitors of nitric oxide synthesis, N-G-monomethylarginine (MMA) and N-G,N-G-dimethylarginine (DMA), are broken down by N-G,N-G-dimethylarginine dimethylaminohydrolase (DDAH) [EC 3.5.3.18], This enzymatic activity is widely distributed in rat tissues, and is correlated with the distribution of free MMA and DMA, and the production of nitric oxide. In this study, immunoblotting analyses with a monoclonal antibody directed towards rat kidney DDAH showed that immunoreactive proteins exist in human pancreas, kidney, and liver. Enzyme activity was definitely detected in these tissues, but the specific activity was lower than that in the corresponding rat tissue. These results are interesting in connection with the idea that the enzyme might play a role as a regulator of nitric oxide generation in human tissues.

引用

页码：237 / 238

页数：2

共 13 条

[1]

Fickling SA, 1993, ENDOTHELIUM, V1, P137

[2] THE METABOLISM OF L-ARGININE AND ITS SIGNIFICANCE FOR THE BIOSYNTHESIS OF ENDOTHELIUM-DERIVED RELAXING FACTOR - CULTURED ENDOTHELIAL-CELLS RECYCLE L-CITRULLINE TO L-ARGININE [J].

HECKER, M ;

SESSA, WC ;

HARRIS, HJ ;

ANGGARD, EE ;

VANE, JR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (21) :8612-8616

[3]

HIBBS JB, 1987, J IMMUNOL, V138, P550

[4]

KAKIMOTO Y, 1970, J BIOL CHEM, V245, P5751

[5] DETECTION OF N(G), N(G)-DIMETHYLARGININE DIMETHYLAMINOHYDROLASE IN THE NITRIC OXIDE-GENERATING SYSTEMS OF RATS USING MONOCLONAL-ANTIBODY [J].

KIMOTO, M ;

TSUJI, H ;

OGAWA, T ;

SASAOKA, K .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 300 (02) :657-662

[6] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[7] METABOLISM OF METHYLARGININES BY HUMAN VASCULATURE - IMPLICATIONS FOR THE REGULATION OF NITRIC-OXIDE SYNTHESIS [J].

MACALLISTER, RJ ;

FICKLING, SA ;

WHITLEY, GS ;

VALLANCE, P .

BRITISH JOURNAL OF PHARMACOLOGY, 1994, 112 (01) :43-48

[8] METABOLISM OF NG,NG-DIMETHYLARGININE AND NG,N'G-DIMETHYLARGININE IN RATS [J].

OGAWA, T ;

KIMOTO, M ;

WATANABE, H ;

SASAOKA, K .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1987, 252 (02) :526-537

[9]

OGAWA T, 1990, J BIOL CHEM, V265, P20938

[10]

OGAWA T, 1989, J BIOL CHEM, V264, P10205

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