MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY - A POWERFUL TOOL FOR THE MASS AND SEQUENCE-ANALYSIS OF NATURAL AND MODIFIED OLIGONUCLEOTIDES

被引：440

作者：

PIELES, U ^{[1
]}

ZURCHER, W ^{[1
]}

SCHAR, M ^{[1
]}

MOSER, HE ^{[1
]}

机构：

[1] CIBA GEIGY AG,CORP ANALYT RES,CH-4002 BASEL,SWITZERLAND

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 14期

关键词：

D O I：

10.1093/nar/21.14.3191

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the analysis and characterization of natural and modified oligonucleotides by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The present technology was highly improved for this class of compounds by using a new matrix, 2,4,6-trihydroxy acetophenone, together with di- and triammonium salts of organic or inorganic acids to suppress peak broadening due to multiple ion adducts. This methodology can be used in combination with time dependent degradation of oligonucleotides by exonucleases as powerful tool to determine sequence compositions.

引用

页码：3191 / 3196

页数：6

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