6-AZAURACIL INHIBITION OF GTP BIOSYNTHESIS IN SACCHAROMYCES-CEREVISIAE

被引：224

作者：

EXINGER, F ^{[1
]}

LACROUTE, F ^{[1
]}

机构：

[1] CNRS,CGM,F-91190 GIF SUR YVETTE,FRANCE

来源：

CURRENT GENETICS | 1992年 / 22卷 / 01期

关键词：

SACCHAROMYCES-CEREVISIAE; IMP DEHYDROGENASE; 6-AZAURACIL; GTP LEVEL;

D O I：

10.1007/BF00351735

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The addition of 6-azauracil to the growth medium causes a strong reduction of the GTP level in the nucleotide pool of Saccharomyces cerevisiae. In-vitro experiments show a strong inhibition of IMP dehydrogenase activity by 6-azaUMP explaining the preceeding effect. PPR2 mutants, previously characterized by an increased sensitivity to 6-azauracil compared to the wild-type, are specifically susceptible to the lowering of the GTP pool, and are able to grow in presence of 6-azauracil when guanine is added to the medium.

引用

页码：9 / 11

页数：3

共 9 条

[1] A STRAIGHTFORWARD RADIOMETRIC TECHNIQUE FOR MEASURING IMP DEHYDROGENASE [J].