MORPHOLOGICAL CHARACTERIZATION OF CARDIOMYOCYTES ISOLATED FROM A TRANSPLANTABLE CARDIAC TUMOR DERIVED FROM TRANSGENIC MOUSE ATRIA (AT-1 CELLS)

被引:76
作者
DELCARPIO, JB
LANSON, NA
FIELD, LJ
CLAYCOMB, WC
机构
[1] LOUISIANA STATE UNIV,MED CTR,DEPT BIOCHEM & MOLEC BIOL,1901 PERDIDO ST,NEW ORLEANS,LA 70112
[2] LOUISIANA STATE UNIV,MED CTR,DEPT ANAT,NEW ORLEANS,LA 70112
[3] INDIANA UNIV,SCH MED,DEPT MED,KRANNERT INST CARDIOL,INDIANAPOLIS,IN 46202
关键词
TISSUE CULTURE; ATRIAL HEART CELLS; CELL DIVISION; MORPHOLOGY; TRANSGENIC MICE;
D O I
10.1161/01.RES.69.6.1591
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We have developed a transplantable tumor lineage derived from transgenic mouse atrial cardiomyocytes that express the SV40 large T oncogene and have named these cardiomyocytes AT-1 cells. In this study, the transplantable tumors, freshly isolated tumor cardiomyocytes, and cultured tumor cardiomyocytes were examined using phase-contrast microscopy, autoradiography, and electron microscopy. The vast majority of the subcutaneous tumor cells, > 90% of the cellular mass of the tumor, exhibited sarcomeric banding. Ultrastructural characteristics typical of in vivo atrial cardiac muscle cells, including well-organized myofibrils, gap junctions, and atrial-specific cytoplasmic granules, were observed in in situ and in freshly isolated AT-1 cells. Those cells that did not contain some form of organized myofibrils were primarily vascular elements, such as endothelial cells. Labeling with [H-3]thymidine indicated that > 90% of cultured AT-1 cells were synthesizing DNA; furthermore, many cells could be seen undergoing cell division. Electron microscopy revealed that the cultured AT-1 cardiomyocytes contained all of the above-described characteristics, including a well-developed transverse tubular system.
引用
收藏
页码:1591 / 1600
页数:10
相关论文
共 20 条
[1]   COMPARATIVE STEREOLOGY OF MOUSE ATRIA [J].
BOSSEN, EH ;
SOMMER, JR ;
WAUGH, RA .
TISSUE & CELL, 1981, 13 (01) :71-77
[2]   IMMUNOCYTOCHEMICAL LOCALIZATION OF ATRIAL NATRIURETIC FACTOR IN THE HEART AND SALIVARY-GLANDS [J].
CANTIN, M ;
GUTKOWSKA, J ;
THIBAULT, G ;
MILNE, RW ;
LEDOUX, S ;
MINLI, S ;
CHAPEAU, C ;
GARCIA, R ;
HAMET, P ;
GENEST, J .
HISTOCHEMISTRY, 1984, 80 (02) :113-127
[3]  
CLAYCOMB WC, 1983, DEV BIOL, V80, P446
[4]   ULTRASTRUCTURAL MORPHOMETRIC ANALYSIS OF CULTURED NEONATAL AND ADULT-RAT VENTRICULAR CARDIAC-MUSCLE CELLS [J].
DELCARPIO, JB ;
CLAYCOMB, WC ;
MOSES, RL .
AMERICAN JOURNAL OF ANATOMY, 1989, 186 (04) :335-345
[5]   LIGHT, SCANNING, AND TRANSMISSION ELECTRON-MICROSCOPY OF A SINGLE POPULATION OF DETERGENT-EXTRACTED CARDIAC MYOCYTES INVITRO [J].
DELCARPIO, JB ;
UNDERWOOD, EG ;
MOSES, RL .
JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE, 1989, 12 (01) :24-28
[6]   DIFFERENTIATION OF SARCOPLASMIC RETICULUM AND T SYSTEM IN DEVELOPING CHICK SKELETAL MUSCLE IN VITRO [J].
EZERMAN, EB ;
ISHIKAWA, H .
JOURNAL OF CELL BIOLOGY, 1967, 35 (2P1) :405-&
[7]  
FERGUSON DG, 1983, ACTA ANAT, V117, P289
[9]   THE ATRIAL MYOCARDIAL-CELLS OF MOUSE HEART - A STRUCTURAL AND STEREOLOGICAL STUDY [J].
FORBES, MS ;
VANNIEL, EE ;
PURDYRAMOS, SI .
JOURNAL OF STRUCTURAL BIOLOGY, 1990, 103 (03) :266-279