CONSTRUCTION OF CLONING VECTORS FOR BACILLUS-THURINGIENSIS

被引：370

作者：

ARANTES, O ^{[1
]}

LERECLUS, D ^{[1
]}

机构：

[1] INST PASTEUR,CNRS,URA 1300,UNITE BIOCHIM MICROBIENNE,25 RUE DOCTEUR ROUX,F-75724 PARIS 15,FRANCE

来源：

GENE | 1991年 / 108卷 / 01期

关键词：

PLASMID COPY NUMBER; GENE EXPRESSION; DELTA-ENDOTOXINS; SEGREGATIONAL STABILITY; REPLICATION REGION; HYDROXYLAMINE MUTAGENESIS; BACILLUS-SUBTILIS;

D O I：

10.1016/0378-1119(91)90495-W

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The replication region of the Bacillus thuringiensis plasmid, pHT1030, was treated with hydroxylamine. Various copy-number mutants were selected and subsequently used to construct shuttle vectors with multiple cloning sites. These recombinant plasmids are very stable and allowed the cloning of a sigma-endotoxin-encoding gene in B. thuringiensis. Comparison between gene expression level and vector copy-number indicated that a plateau in sigma-endotoxin production is reached with a copy-number of about fifteen per equivalent chromosome.

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页码：115 / 119

页数：5

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