SINGLE CELL ASSAY OF EXOCYTOSIS FROM PANCREATIC-ISLET B-CELLS

被引：61

作者：

GILLIS, KD

MISLER, S

机构：

[1] WASHINGTON UNIV,JEWISH HOSP ST LOUIS,DEPT MED,DIV RENAL,YALEM RES 713,216 S KINGSHIGHWAY,ST LOUIS,MO 63110

[2] WASHINGTON UNIV,DEPT CELL BIOL PHYSIOL,ST LOUIS,MO 63110

[3] WASHINGTON UNIV,DEPT ELECT ENGN,ST LOUIS,MO 63110

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1992年 / 420卷 / 01期

关键词：

DEPOLARIZATION-SECRETION COUPLING; MEMBRANE CAPACITANCE; PANCREATIC B-CELL; EXOCYTOSIS; ENDOCYTOSIS;

D O I：

10.1007/BF00378654

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

We have measured changes in membrane capacitance (DELTA-C(m)) in pancreatic B cells as a single cell assay of insulin secretion. Evidence that depolarization evoked DELTA-C(m) reflects exocytosis includes it's voltage, temperature and Ca2+o dependence. Decreases in C(m) presumably reflecting endocytosis, occur on a variable time scale. Two features that make B cells unique among excitable cells are the large magnitude of DELTA-C(m), when normalized to Ca2+ current, and the rapid "fatigue" of the response for even minimal stimulation, perhaps due to the depletion of a readily releasable pool of vesicles.

引用

页码：121 / 123

页数：3

共 13 条

[1] SINGLE CELL ASSAY OF EXOCYTOSIS FROM ADRENAL CHROMAFFIN CELLS USING PERFORATED PATCH RECORDING [J].