MCM3 COMPLEX REQUIRED FOR CELL-CYCLE REGULATION OF DNA-REPLICATION IN VERTEBRATE CELLS

被引：246

作者：

MADINE, MA ^{[1
]}

KHOO, CY ^{[1
]}

MILLS, AD ^{[1
]}

LASKEY, RA ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,DEPT ZOOL,CAMBRIDGE CB2 3EJ,ENGLAND

来源：

NATURE | 1995年 / 375卷 / 6530期

基金：

英国惠康基金;

关键词：

D O I：

10.1038/375421a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

AN intact nuclear membrane restricts DNA replication to only one round in each cell cycle, apparently by excluding an essential replication-licensing factor throughout interphase(1-5). A family of related yeast replication proteins, MCM2, 3 and 5 (also called, after cell-division cycle, CDC46), resemble licensing factor, entering the nucleus only during mitosis(6-8). We have cloned a Xenopus homologue of MCM3 (XMCM3) and raised antibodies against expressed protein, Immunodepletion of Xenopus egg extracts removes a complex of MCM2, 3 and 5 homologues and inhibits replication of Xenopus sperm nuclei or permeable G2 HeLa nuclei. However, G1 HeLa nuclei still replicate efficiently. Mock-depleted extracts replicate all three templates. XMCM3 accumulates in nuclei before replication but anti-XMCM3 staining decreases during replication. These results can explain why replicated nuclei are unable to reinitiate replication in a single cell cycle.

引用

页码：421 / 424

页数：4

共 28 条

[1] INITIATION OF DNA-REPLICATION IN NUCLEI AND PURIFIED DNA BY A CELL-FREE-EXTRACT OF XENOPUS EGGS [J].