EFFICIENT TRANSACTIVATION BY THE HIV-2 TAT PROTEIN REQUIRES A DUPLICATED TAR RNA STRUCTURE

被引:73
作者
BERKHOUT, B
GATIGNOL, A
SILVER, J
JEANG, KT
机构
[1] Laboratory of Molecular Microbiology, National Institute of Allergy, Infectious Diseases, Bethesda
基金
美国国家卫生研究院;
关键词
D O I
10.1093/nar/18.7.1839
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Human immunodeficiency viruses HIV-1 and HIV-2 encode a Tat protein that activates transcription from the long terminal repeats. The target for trans-activation is termed the trans-acting responsive (TAR) element. TAR has an extensively folded RNA secondary structure and is present at the 5′ end of all viral mRNAs. Considerable similarities exist between both Tat and TAR of the two viruses. The TAR element of HIV-2 (TAR-2) resembles a tandem duplication of the TAR-1 hairpin structure. Tat-2 conserves many of the protein domains in Tat-1, although it is slightly larger than its counterpart. Given the similarity between the two Tat proteins, it is somewhat unexpected that HIV-2 Tat (Tat-2) only poorly activates the heterologous TAR-1 element. Here, we tested whether the duplicated structure of TAR-2 is required for full Tat-2 activity. We show that the addition of a second TAR hairpin to TAR-1 increased its Tat-2 responsiveness by 3-fold. © 1990 Oxford University Press.
引用
收藏
页码:1839 / 1846
页数:8
相关论文
共 42 条
[1]   TRANS-ACTIVATOR GENE OF HUMAN T-LYMPHOTROPIC VIRUS TYPE-III (HTLV-III) [J].
ARYA, SK ;
GUO, C ;
JOSEPHS, SF ;
WONGSTAAL, F .
SCIENCE, 1985, 229 (4708) :69-73
[2]   HUMAN IMMUNODEFICIENCY VIRUS TYPE-2 LONG TERMINAL REPEAT - ANALYSIS OF REGULATORY ELEMENTS [J].
ARYA, SK ;
GALLO, RC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (24) :9753-9757
[3]  
AYRA SK, 1987, NATURE, P548
[4]   TAT TRANS-ACTIVATES THE HUMAN IMMUNODEFICIENCY VIRUS THROUGH A NASCENT RNA TARGET [J].
BERKHOUT, B ;
SILVERMAN, RH ;
JEANG, KT .
CELL, 1989, 59 (02) :273-282
[6]   INVITRO MUTAGENESIS OF THE HUMAN T-CELL LEUKEMIA-VIRUS TYPE-1 AND TYPE-2 TAX GENES [J].
CANN, AJ ;
ROSENBLATT, JD ;
WACHSMAN, W ;
CHEN, ISY .
JOURNAL OF VIROLOGY, 1989, 63 (03) :1474-1479
[7]   STRUCTURE OF SIMIAN IMMUNODEFICIENCY VIRUS REGULATORY GENES [J].
COLOMBINI, S ;
ARYA, SK ;
REITZ, MS ;
JAGODZINSKI, L ;
BEAVER, B ;
WONGSTAAL, F .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (13) :4813-4817
[8]   TRANSACTIVATION OF HUMAN-IMMUNODEFICIENCY-VIRUS OCCURS VIA A BIMODAL MECHANISM [J].
CULLEN, BR .
CELL, 1986, 46 (07) :973-982
[9]   THE TRANSACTIVATOR GENE OF THE HUMAN T-CELL LYMPHOTROPIC VIRUS TYPE-III IS REQUIRED FOR REPLICATION [J].
DAYTON, AI ;
SODROSKI, JG ;
ROSEN, CA ;
GOH, WC ;
HASELTINE, WA .
CELL, 1986, 44 (06) :941-947
[10]   MODULARITY IN PROMOTERS AND ENHANCERS [J].
DYNAN, WS .
CELL, 1989, 58 (01) :1-4