STRUCTURE-FUNCTION ANALYSIS OF EPIDERMAL GROWTH-FACTOR - SITE DIRECTED MUTAGENESIS AND NUCLEAR-MAGNETIC-RESONANCE

被引：52

作者：

DUDGEON, TJ

COOKE, RM

BARON, M

CAMPBELL, ID

EDWARDS, RM

FALLON, A

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,S PARKS RD,OXFORD OX1 3QU,ENGLAND

[2] BRITISH BIOTECHNOL LTD,OXFORD OX4 5LY,ENGLAND

来源：

FEBS LETTERS | 1990年 / 261卷 / 02期

关键词：

Epidermal growth factor; human; Protein NMR; Site directed mutagenesis;

D O I：

10.1016/0014-5793(90)80600-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of leucine-47 in determining the structure and activity of human epidermal growth factor was examined using site-directed mutagenesis. Wild type protein and four variants in which Leu47 was replaced by valine, glutamate, aspartate and alanine were produced from yeast. 1H NMR experiments demonstrated that substitution of Leu47 had little effect on the protein structure. The observed reduction in receptor binding affinity caused by the substitutions could thus be attributed to perturbation of a residue directly involved in receptor interactions. © 1990.

引用

页码：392 / 396

页数：5

共 20 条

[1] TRANSFORMATION OF YEAST BY A REPLICATING HYBRID PLASMID [J].