THE INTRINSIC MIGRATORY CAPACITY OF MEMORY T-CELLS CONTRIBUTES TO THEIR ACCUMULATION IN RHEUMATOID SYNOVIUM

被引:78
作者
CUSH, JJ [1 ]
PIETSCHMANN, P [1 ]
OPPENHEIMERMARKS, N [1 ]
LIPSKY, PE [1 ]
机构
[1] UNIV TEXAS,SW MED CTR,ARTHRITIS RES CTR,DALLAS,TX 75235
来源
ARTHRITIS AND RHEUMATISM | 1992年 / 35卷 / 12期
关键词
D O I
10.1002/art.1780351206
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective. Mechanisms controlling the infiltration of T cells into rheumatoid synovium have not been fully characterized. These studies were undertaken to investigate the relationship between T cell phenotype and migratory capacity, so as to elucidate mechanisms that might contribute to the accumulation of T cells at inflammatory sites. Methods. The characteristics of in vivo migrating cells were studied by dual-immunofluorescence FACS (fluorescence-activated cell sorter) analysis of rheumatoid synovial and peripheral blood T cells. Migratory cells were also characterized using a recently developed in vitro assay, wherein peripheral blood T lymphocytes (PBTL) with the capacity to migrate through endothelial cell monolayers were retrieved and assessed. Results. Migratory CD4+ T cells from rheumatoid arthritis (RA) and normal individuals were characterized as being CD45RA-, CD29bright, CD11a(bright), L-selectin-, CD54+, and CD58+. Migrating RA PBTL (compared with normal PBTL), however, were significantly enriched in activated HLA-DR+ T cells. RA synovial tissue lymphocytes exhibited a similar phenotype, but with decreased surface density of CD4 and an increase in HLA-DR and VLA-1. RA synovial lymphocytes exhibited a 2-3-fold increase in migratory capacity over normal and RA PBTL. Conclusion. These studies demonstrate the inherent migratory proficiency of CD4+ T cells that express a memory phenotype (CD29bright, CD11a(bright), and CD58+). In addition, enhanced transendothelial migration was observed for CD4+ T cells that were CD54+ and L-selectin-. These studies demonstrate that the migratory patterns of circulating lymphocytes may be correlated with their surface phenotype and that the intrinsic migratory capacity of memory T cells is one component contributing to their accumulation in the rheumatoid synovium.
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页码:1434 / 1444
页数:11
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