COMPLETE RECONSTITUTION OF MOUSE-LIVER WITH XENOGENEIC HEPATOCYTES

被引：168

作者：

RHIM, JA

SANDGREN, EP

PALMITER, RD

BRINSTER, RL

机构：

[1] UNIV PENN,SCH VET MED,DEPT ANIM BIOL,PHILADELPHIA,PA 19104

[2] UNIV PENN,SCH VET MED,DEPT PATHOL & LAB MED,PHILADELPHIA,PA 19104

[3] UNIV WASHINGTON,HOWARD HUGHES MED INST,SEATTLE,WA 98195

[4] UNIV WASHINGTON,DEPT BIOCHEM,SEATTLE,WA 98195

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 11期

关键词：

LIVER CELL TRANSPLANTATION; LIVER REGENERATION; XENOGENEIC TRANSPLANTATION; TRANSGENIC;

D O I：

10.1073/pnas.92.11.4942

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have developed a system for studying hepatocellular growth potential in which liver cells are introduced into the diseased livers of albumin-urokinase (Alb-uPA) transgenic mice. To use this system to study xenogeneic cell transplantation, rat liver cells were introduced into immunotolerant Alb-uPA transgenic mice. In regenerated recipient livers, up to 100% of hepatocellular gene expression was of rat origin, demonstrating the creation of a functional mouse liver in which parenchyma is derived from xenogeneic (rat) hepatocytes. Immunotolerant Alb-uPA transgenic mice provide a tool for studying hepatocellular biology of any species, including humans, in a controlled experimental setting.

引用

页码：4942 / 4946

页数：5

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