PEROXISOMAL ACYL COENZYME-A OXIDASE IS A RATE-LIMITING ENZYME IN A VERY-LONG-CHAIN FATTY-ACID BETA-OXIDATION SYSTEM

被引：27

作者：

AOYAMA, T ^{[1
]}

SOURI, M ^{[1
]}

KAMIJO, T ^{[1
]}

USHIKUBO, S ^{[1
]}

HASHIMOTO, T ^{[1
]}

机构：

[1] SHINSHU UNIV, SCH MED, DEPT PEDIAT, MATSUMOTO, NAGANO 390, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 201卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.1879

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The contents of peroxisomal fatty acid beta-oxidation enzymes in three rat hepatoma cell lines,i.e.,H4IIEC3 (H4), N1S1, and McA-KH7777 (H7), were measured by immunoblot analysis, and a significant difference in acyl-coenzyme A oxidase (AOX) content became evident. These cell lines were respectively infected with a recombinant virus to express significant amounts of AOX protein. The expressed AOX mainly localized in organelle, supposing peroxisomes, and was catalytically active. The cDNA-expression in H4, N1S1, and H7 eels enhanced 2.6-, 2.2-, and 1.0-fold beta-oxidation activity of lignoceric acid, respectively. The enhancement in H4 and NISI cells suggests that AOX is a rate-limiting enzyme in the very-long-chain fatty acid beta-oxidation system,in these cell lines. (C) 1994 Academic Press, Inc.

引用

页码：1541 / 1547

页数：7

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