ANGIOGENESIS AND THE BLOOD-BRAIN-BARRIER IN INTRACEREBRAL SOLID AND CELL-SUSPENSION GRAFTS

被引:11
作者
AKALAN, N [1 ]
GRADY, MS [1 ]
机构
[1] UNIV WASHINGTON, DEPT NEUROSURG, SEATTLE, WA USA
来源
SURGICAL NEUROLOGY | 1994年 / 42卷 / 06期
关键词
ANGIOGENESIS; BLOOD-BRAIN BARRIER; NEURAL TRANSPLANTATION;
D O I
10.1016/0090-3019(94)90082-5
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Solid and suspension grafts of fetal central nervous system (CNS) tissue rapidly reform an intact blood-brain barrier (BBB), whereas solid grafts of peripheral nervous system (PNS) tissue fail to establish a BBB as detected by horseradish peroxide (HRP) leakage, administrated intravenously. We examined the acute changes in the BBB after grafting of fetal CNS tissue in solid and suspension form and superior cervical ganglion (SCG) and PNS tissue in the same manner. Adult rats (n = 20) received fetal (day 14-15) forebrain grafts (either solid or cell suspension) to their rostral corpus callosum bilaterally. A second group (n = 20) received SCG solid and eel suspension grafts at the same coordinates with the same technique. The animals were killed on first, third, seventh, and tenth days after grafting. Intravenous HRP (Sigma, type VI, 75 mg/5-g rat) was given 1 hour before perfusion with mixed aldehydes. Fifty-micron coronal sections were examined for the presence and location of the graft by cresyl violet and AChE staining and Mesulam's TMB method to detect HRP leakage. HRP leakage was detected in the parenchyma in all groups on the first and the third days post-transplantation indicating a disrupted BBB. No HRP reaction was seen at days 7 and 10 in groups receiving fetal forebrain tissue whether solid or cell suspension. Solid grafts of SCG consistently demonstrated HRP leakage from the first through the tenth day. However, cell suspension of SCG established a BBB by 7 days. These results suggest that within the solid grafts of CNS and PNS tissue, the permeability of the vessels is dictated by the transplanted tissue itself When cell suspensions of the same tissue are introduced, host CNS tissue dominates as the local environment resulting in non-leaky vasculature within the graft.
引用
收藏
页码:517 / 522
页数:6
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