AFFINITY-CHROMATOGRAPHY OF HUMAN PLASMA GELSOLIN WITH POLYPHOSPHATE COMPOUNDS ON IMMOBILIZED CIBACRON BLUE F3GA

Human plasma gelsolin was specifically eluted from a Cibacron Blue F3GA column with 1 mM adenosine, guanosine, cytidine and uridine di- and triphosphates, except for cytidine 5′-diphosphate. Inorganic polyphosphates also eluted gelsolin, but neither nicotinamide adenine dinucleotide nor nicotinamide adenine dinucleotide phosphate did so. The results suggest that a terminal pyrophosphate structure might be essential for the specific elution. After elution with 1 mM adenosine 5′-triphosphate, additional gelsolin was eluted by washing the column with a high salt concentration. This indicates that human plasma gelsolin may bind to Cibacron Blue F3GA in at least two different ways. © 1990.