GENETIC-ANALYSIS OF IONIZING-RADIATION SENSITIVE MUTANTS OF CULTURED MAMMALIAN-CELL LINES

被引：89

作者：

JEGGO, PA ^{[1
]}

TESMER, J ^{[1
]}

CHEN, DJ ^{[1
]}

机构：

[1] UNIV CALIF LOS ALAMOS SCI LAB,GENET GRP,LOS ALAMOS,NM 87544

来源：

MUTATION RESEARCH | 1991年 / 254卷 / 02期

关键词：

IONIZING RADIATION SENSITIVE MUTANTS; MAMMALIAN CELL LINES; CULTURED; HYBRIDS; CONSTRUCTION; HAT; OUABAIN;

D O I：

10.1016/0921-8777(91)90003-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The genetic diversity of a range of ionising radiation sensitive mutants of cultured mammalian cell lines has been examined. Hybrids were constructed from suitably marked diploid cells by cell fusion and selected using resistance to HAT and ouabain. Hybrids were examined for ploidy and gamma-ray sensitivity. The data suggest that at least 8 and possibly 9 complementation groups exist which confer sensitivity to ionising radiation. Mutants in at least 3 distinct complementation groups have a reduced ability to rejoin DNA double-strand breaks.

引用

页码：125 / 133

页数：9

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