IPGMAKER - A PROGRAM FOR IBM-COMPATIBLE PERSONAL COMPUTERS TO CREATE AND TEST RECIPES FOR IMMOBILIZED PH GRADIENTS

被引：20

作者：

ALTLAND, K

机构：

来源：

ELECTROPHORESIS | 1990年 / 11卷 / 02期

关键词：

D O I：

10.1002/elps.1150110207

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The program “IPGMAKER” is a computational aid for creating and testing recipes for near‐linear immobilized pH gradients. It was written for fast IBM personal computers (with a Type 80386 processor and 80387 coprocessor) and compatibles equipped with a VGA, EGA or Hercules (mono) graphics card. The program is limited to the use of up to 10 acids and/or bases, and to ranges spanning between pH 2 and 12. The resulting recipes are presented either as final concentrations in the 2 chambers of a mixing device for linear gradients or as volumes from 0.2 moles/L stock solutions adjusted to a user‐defined average buffering power. One of the subroutines determines the pH, gradient slope and buffering capacity at any location of the gradient and includes a facility to estimate the pI of proteins from the composition of their primary structure. Copyright © 1990 VCH Verlagsgesellschaft mbH

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页码：140 / 147

页数：8

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