HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROCHEMICAL ASSAY FOR MONITORING THE FORMATION OF 8-OXO-7,8-DIHYDROADENINE AND ITS RELATED 2'-DEOXYRIBONUCLEOSIDE

被引：66

作者：

BERGER, M

ANSELMINO, C

MOURET, JF

CADET, J

机构：

[1] Laboratoires de Chimie, Departement de Recherche Fondamentale, Centre d'Etudes Nucléaires, F-38041, Grenoble

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1990年 / 13卷 / 05期

关键词：

D O I：

10.1080/01483919008049222

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive method involving the combined use of reversed-phase high-performance liquid chromatography and electrochemical detection has been developed for monitoring the formation of 8-oxo-7,8-dihydro-adenine and 8-oxo-7,8-dihydro-2‘-deoxyadenosine. The limit of detection for both compounds is close to 0.2 ng under optimised conditions of detection at an oxidation potential of 850 mV. This assay allows the quantitative measurement of the hydroxylated purine components in aqueous solutions of adenine and 2’-deoxyadenosine exposed to a dose of ionizing radiation as low as 2 Gray (Gy). © 1990, Taylor & Francis Group, LLC. All rights reserved.

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页码：929 / 940

页数：12

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