LIBRARY SUBTRACTION OF INVITRO CDNA LIBRARIES TO IDENTIFY DIFFERENTIALLY EXPRESSED GENES IN SCRAPIE INFECTION

被引：160

作者：

DUGUID, JR

DINAUER, MC

机构：

[1] BOSTON UNIV,SCH MED,DEPT BIOCHEM,BOSTON,MA 02118

[2] BOSTON UNIV,SCH MED,DEPT NEUROL,BOSTON,MA 02118

[3] CHILDRENS HOSP MED CTR,DIV HEMATOL ONCOL,BOSTON,MA 02115

[4] HARVARD UNIV,SCH MED,DANA FARBER CANC INST,BOSTON,MA 02115

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 09期

关键词：

D O I：

10.1093/nar/18.9.2789

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a system where double-stranded cDNA can be amplified using a synthetic ollgonucleotide primer and the polymerase chain reaction, generating cDNA libraries in vitro. Using a library subtraction strategy (1), scrapie and control brain in vitro cDNA libraries were used to identify sequences whose expression is modulated In scrapie infection. One of these sequences represents β-2 microglobulin, while the other two have not been previously described. The use of in vitro libraries offers increased speed and efficiency of construction, and their subtraction is more efficient and powerful, compared with the previous system (1). © 1990 Oxford University Press.

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页码：2789 / 2792

页数：4

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