CHARACTERIZATION OF BETA-ADRENOCEPTORS ON CULTURED ENDOTHELIAL-CELLS BY RADIOLIGAND BINDING

被引：27

作者：

AHMAD, S ^{[1
]}

CHRETIEN, P ^{[1
]}

DANIEL, EE ^{[1
]}

SHEN, SH ^{[1
]}

机构：

[1] MCMASTER UNIV,SMOOTH MUSCLE RES PROGRAM,HAMILTON L8N 3Z5,ONTARIO,CANADA

来源：

LIFE SCIENCES | 1990年 / 47卷 / 25期

关键词：

D O I：

10.1016/0024-3205(90)90276-W

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The properties of β-adrenoceptors present on the cultured bovine pulmonary artery endothelial cells were studied by radioligand binding. These cells contain a high density of β-adrenoceptors (∼ 16,000 receptors/cell) having high affinity (Kd 18 pM) for 125I-iodocyanopindolol (ICYP). Competition binding experiments suggested the presence of more than two subtype of β-adrenoceptors. 25% of the total population of receptors was found to be of β1-type. The remaining 75% represented a mixed population containing what is suggested to be a mixture of β2- and atypical β-adrenoceptors. © 1990.

引用

页码：2365 / 2370

页数：6

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