DEVELOPMENT OF HUMAN CHROMOSOME-SPECIFIC PCR PRIMERS FOR CHARACTERIZATION OF SOMATIC-CELL HYBRIDS

被引：51

作者：

ABBOTT, C ^{[1
]}

POVEY, S ^{[1
]}

机构：

[1] UNIV LONDON UNIV COLL,MRC,HUMAN BIOCHEM GENET UNIT,LONDON NW1 2HE,ENGLAND

来源：

GENOMICS | 1991年 / 9卷 / 01期

关键词：

D O I：

10.1016/0888-7543(91)90222-Z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The human chromosome complement of somatic cell hybrids must be assessed each time the hybrids are grown in culture. We have developed a panel of human-specific oligonucleotide primers for genes that have been mapped to each of the autosomes and to the X chromosome. These primers enable the human chromosome content of hybrids to be assessed rapidly by PCR. The sequence of the primers is presented together with the appropriate conditions for human DNA-specific amplification for each pair. © 1991.

引用

页码：73 / 77

页数：5

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