GENOTYPIC ANALYSIS OF MULTIPLE LOCI IN SOMATIC-CELLS BY WHOLE GENOME AMPLIFICATION

被引：27

作者：

BARRETT, MT

REID, BJ

JOSLYN, G

机构：

[1] UNIV WASHINGTON,DEPT MED RG24,SEATTLE,WA 98195

[2] FRED HUTCHINSON CANC RES CTR,DIV PUBL HLTH SCI,SEATTLE,WA 98104

来源：

NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 17期

关键词：

D O I：

10.1093/nar/23.17.3488

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To screen multiple loci in small purified samples of diploid and aneuploid cells a PCR-based technique of whole genome amplification was adapted to the study of somatic lesions, DNA samples from different numbers of flow-sorted diploid and aneuploid cells from biopsies were amplified with a degenerate 15mer primer, Aliquots of these reactions were then used in locus-specific reactions using a single round of PCR cycles with individual sets of primers representing polymorphic markers for different regions. As a result, polymorphic markers for different chromosomal regions, including VNTRs and dinucleotide repeats, can be used to perform up to 30 locus-specific PCR assays with a single sample obtained from fewer than 1000 cells.

引用

页码：3488 / 3492

页数：5

共 20 条

[1]

BLOUNT PL, 1991, CANCER RES, V51, P5482

[2] CLONAL ORDERING OF 17P AND 5Q ALLELIC LOSSES IN BARRETT DYSPLASIA AND ADENOCARCINOMA [J].

BLOUNT, PL ;

MELTZER, SJ ;

YIN, J ;

HUANG, Y ;

KRASNA, MJ ;

REID, BJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (08) :3221-3225

[3]

BLOUNT PL, 1994, CANCER RES, V54, P2292

[4] LOSS OF HETEROZYGOSITY INVOLVING THE APC AND MCC GENETIC-LOCI OCCURS IN THE MAJORITY OF HUMAN ESOPHAGEAL CANCERS [J].

BOYNTON, RF ;

BLOUNT, PL ;

YIN, J ;

BROWN, VL ;

HUANG, Y ;

TONG, Y ;

MCDANIEL, T ;

NEWKIRK, C ;

RESAU, JH ;

RASKIND, WH ;

HAGGITT, RC ;

REID, BJ ;

MELTZER, SJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (08) :3385-3388

[5] MUTATION IN THE DNA MISMATCH REPAIR GENE HOMOLOG HMLH1 IS ASSOCIATED WITH HEREDITARY NONPOLYPOSIS COLON-CANCER [J].

BRONNER, CE ;

BAKER, SM ;

MORRISON, PT ;

WARREN, G ;

SMITH, LG ;

LESCOE, MK ;

KANE, M ;

EARABINO, C ;

LIPFORD, J ;

LINDBLOM, A ;

TANNERGARD, P ;

BOLLAG, RJ ;

GODWIN, AR ;

WARD, DC ;

NORDENSKJOLD, M ;

FISHEL, R ;

KOLODNER, R ;

LISKAY, RM .

NATURE, 1994, 368 (6468) :258-261

[6] IDENTIFICATION OF A CHROMOSOME-18Q GENE THAT IS ALTERED IN COLORECTAL CANCERS [J].

FEARON, ER ;

CHO, KR ;

NIGRO, JM ;

KERN, SE ;

SIMONS, JW ;

RUPPERT, JM ;

HAMILTON, SR ;

PREISINGER, AC ;

THOMAS, G ;

KINZLER, KW ;

VOGELSTEIN, B .

SCIENCE, 1990, 247 (4938) :49-56

[7] THE HUMAN MUTATOR GENE HOMOLOG MSH2 AND ITS ASSOCIATION WITH HEREDITARY NONPOLYPOSIS COLON-CANCER [J].

FISHEL, R ;

LESCOE, MK ;

RAO, MRS ;

COPELAND, NG ;

JENKINS, NA ;

GARBER, J ;

KANE, M ;

KOLODNER, R .

CELL, 1993, 75 (05) :1027-1038

[8] P53 MUTATIONS IN HUMAN CANCERS [J].

HOLLSTEIN, M ;

SIDRANSKY, D ;

VOGELSTEIN, B ;

HARRIS, CC .

SCIENCE, 1991, 253 (5015) :49-53

[9] FREQUENT INACTIVATION OF THE RETINOBLASTOMA ANTIONCOGENE IS RESTRICTED TO A SUBSET OF HUMAN TUMOR-CELLS [J].

HOROWITZ, JM ;

PARK, SH ;

BOGENMANN, E ;

CHENG, JC ;

YANDELL, DW ;

KAYE, FJ ;

MINNA, JD ;

DRYJA, TP ;

WEINBERG, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (07) :2775-2779

[10]

HUANG Y, 1992, CANCER RES, V52, P6525

← 1 2 →